1. Introduction1.1Proteases and their uses: Proteases or proteolytic enzymes areenzymes that are seen to have multiple biological applications in plants andanimals, such as germination, inflammatory processes, complement activation andmany more. They can be divided into two broad subcategories, namely exotoxins and endotoxins. For commercial purposes, proteasesare used in leather, food and textile industries.
Of both classes of proteases,endopeptidases have much more significance in industrial use. They have thefollowing applications:· Processingof chymosin in cheese preparation · Soysauce preparation · Meattenderization· Asan alternative to chemical detergents in the leather industry 1.2Isolation from plant sources over microbial sources: For industrial purposes, proteases are usuallyisolated from microbial sources. But of recent, plant sources have showedpromise due to a variety of factors, such as their broad substrate specificity,allowing for more flexible substrate preparations for varied plant sources.
Theyalso have a more wide range of permissible temperature, pH and other factorssuch as the presence of organic compounds. Proteases are seen to be at a highlevel especially during and post germination in seeds. This is due to the factthat these enzymes play an important role in the various biochemical mechanismsinvolved in germination and also in the initial stages of development. Of plantseeds, legume seeds have higher levels of globulin and albumin storageproteins, which are used for the nourishment of the seedling.
Due to the abovefactors, the following investigation of comparing the protease content inmultiple samples has been performed. 2. Reviewof Literature2.1 In 2013,Ranajit Kumar Shaha and Shyam Sundar Shaha studied and compared the germinatingconditions and protease activity in multiple leguminous samples including blackgram, green gram, etc. They further performed time course studies for the sameand obtained an outline of when best to perform protease iisolation for maximumyield.