1. used for the nourishment of the seedling.

1. Introduction

Proteases and their uses:      

            Proteases or proteolytic enzymes are
enzymes that are seen to have multiple biological applications in plants and
animals, such as germination, inflammatory processes, complement activation and
many more. They can be divided into two broad subcategories, namely exotoxins and endotoxins.

            For commercial purposes, proteases
are used in leather, food and textile industries. Of both classes of proteases,
endopeptidases have much more significance in industrial use. They have the
following applications:

of chymosin in cheese preparation

sauce preparation


an alternative to chemical detergents in the leather industry


Isolation from plant sources over microbial sources:

          For industrial purposes, proteases are usually
isolated from microbial sources. But of recent, plant sources have showed
promise due to a variety of factors, such as their broad substrate specificity,
allowing for more flexible substrate preparations for varied plant sources. They
also have a more wide range of permissible temperature, pH and other factors
such as the presence of organic compounds.

            Proteases are seen to be at a high
level especially during and post germination in seeds. This is due to the fact
that these enzymes play an important role in the various biochemical mechanisms
involved in germination and also in the initial stages of development. Of plant
seeds, legume seeds have higher levels of globulin and albumin storage
proteins, which are used for the nourishment of the seedling. Due to the above
factors, the following investigation of comparing the protease content in
multiple samples has been performed.


2. Review
of Literature

2.1 In 2013,
Ranajit Kumar Shaha and Shyam Sundar Shaha studied and compared the germinating
conditions and protease activity in multiple leguminous samples including black
gram, green gram, etc. They further performed time course studies for the same
and obtained an outline of when best to perform protease iisolation for maximum