Abstract: The purpose of this lab experiment is to observe the impact of osmosis in cells.
and also how osmosis works and what are the different effects of different kinds of solutions to cells. In this experiment, we utilized sheep blood and varying centralizations of NaCl arrangement, of 0.9 % Nacl , 0.15 % Nacl and distilled water. Three setups were made where in each set up was presented to a particular centralization of NaCl arrangement.
All setups were watched for few minutes under the microscope and every setup delivered distinctive outcomes. In this activity it was discovered that the control of the centralization of Solutions influences osmosis in red blood cells. This experiment is focus on how cell reacts during each solution added to blood cells.
During the experiment you will also notice that the higher the solute concentration in the solution the greater its water potential will be. the main purpose of this experiment was to determine which direction substance will flow. We also test the movement of solutes from area higher concentration to the area of lower concentration. Introduction: Molecules are in constantly motion, they tend to move from areas of high concentration, to areas of low concentration. This broad principle is divided into two categories: diffusion and osmosis.
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There are two type of membrane transport , active transport and passive transport. Active transport is movement against the gradient from low to high concentration. Passive transport is solute along with the concentration gradient with higher to lower concentration therefore no energy needed. Osmosis is the movement of solvent molecules through a selectively permeable membrane into a region of higher solute concentration, to equalize the solute concentrations on the two sides. The term osmosis also defined by the movement of a solvent through a semipermeable membrane into a solution of higher solute concentration that trends to equalize the concentrations of solute on the membrane ( marieb webster) .
Another key word in this experiment is diffusion. Osmosis is movement of water from higher to lower concentration of solvent or lower to higher concentration of solute. There are 3 types of solution that can occur in the body which is hypertonic, hypotonic and isotonic.
Hypertonic solution is higher solute concentration to less water concentration. Hypotonic solution is lower solute concentration and more water. Isotonic solution in which water and solute molecules are equal in concentration. Diffusion is the net movement of molecules down their concentration gradient. There are many different factors that can affect osmosis.
Such as a temperature, time and size of concentration gradient. The higher the temperature osmosis would move much faster than the lower temperature does. The purpose of this lab is to observe the physical effects of osmosis and diffusion and determine if it actually, takes place. We predict that after each solution added to blood cells, we would have different result according to concentration of solute. We hypo these that molecules tend to move along gradient higher to lower concentration, therefore water will diffuse across semipermeable membrane. From higher concentration of water to the lower concentration of water.
Materials: Microscope 5 glass slides Pen or marker Small plastic cover (to cover solution on slide) Distilled water Sheep blood Nacl solution (sodium chloride 0.9% and 0.15%) Goggles & gloves Dropper Toothpick or wooden stick (4 or more) Clean surface.
