In biology there are two types of organisms, prokaryotes (e.g. bacteria) and eukaryotes. Each of these different organism goes through a process known as transcription. Transcription is the process where a strand of RNA gets to attach and fit perfectly with a single strand of DNA. The transcription process uses the enzyme RNA Polymerase to facilitate the duplication or copying of the DNA information into its products such as mRNA, tRNA, and rRNA. With the process of Transcription, prokaryotes and eukaryotes undergo control and regulation as affected by several factors. For prokaryotes such as bacteria, transcription occurs in the cytoplasm of the cell. In the cytoplasm, during the process of transcription, the RNA polymerase attaches to the DNA nucleotides at the starting point of the gene. At this point of the DNA, nucleotides would be known as the promoter region. This is where the DNA strand had its sequences of a gene. The promoter is the first step that makes the process of transcription to start. Once this step is done, the RNA polymerase ‘untwists’ the DNA double helix in order for it to form the transcription bubble. A major difference between prokaryotes and eukaryotes transcription is at the DNA separation. In prokaryotes, the DNA does not separate from the RNA for the reason that bacteria is missing the nucleus and its membrane that separates the DNA and the product RNA. The control of transcription in the prokaryotic cells are known as Transcription factors and the regulatory region of a gene is called operon. These would help the bacteria cells to respond quickly to environmental changes. The regulatory regions have promoters for RNA polymerase as well as the DNA region where regulatory proteins attach. These regulatory proteins attach to the DNA to either start or stop the process of transcription. For the case of bacteria cells, the regulatory proteins stop the process of transcription. For the eukaryotic cells, transcription takes places in the nucleus. The transcription in prokaryotes are relatively similar to those in the bacteria’s transcription. The first thing it does is the RNA polymerase attaches to the DNA nucleotides known as the promoter region which is where the DNA sequences of the genes are. Then the RNA polymerase unrolls again from the DNA helix to form the transcription bubble. The transcription bubble is what keeps the double strand of DNA separated. The control transcription in eukaryotes have multiple regions for one gene. This would allow the eukaryotes to make different types of proteins from a single gene. Unlike bacteria cells, eukaryotes have more of a flexibility to react at the changing of the environment. The regulatory proteins in eukaryotes play as transcription factors which it works to initiate or start the process of transcriptions. In addition, the reproduction of the prokaryotes and eukaryotes is what makes the difference due to the life’s history of the cells. In bacterial cells, the way life history is developed is by reproduction process of binary fission, a form of asexual reproduction. In the process of binary fission the cells replicate into two daughter cells. These cells are identical from their parents and reproduce again into two new identical cells. The reason behind why prokaryotes live a shorter lifespan is that they have more of a simple process of replication than eukaryotes. Eukaryotes have complex properties of reproduction, growth and adaptation making them survive in different types of environments and longer lifespan than prokaryotes.